Three-stage single-cell RNA sequencing workflow showing tissue dissociation and droplet isolation, barcoded cDNA library preparation, and high-throughput sequencing.

A Beginner's Guide to Single-Cell Transcriptomics

A practical map of how single-cell RNA sequencing moves from isolated cells and barcoded molecules to expression matrices and biological interpretation.

2019 · Hojae Lee, Hengshi Yu, Joshua D. Welch